Benthamiana rhapsody

The Australian outback circa 1994.
Nicotiana benthamiana aka benthi — the Australian wonder plant. LAB is the commonly used laboratory line is compared here to wild accessions. Source: Bally et al. 2015.
Collection sites (pins) of Nicotiana benthamiana accessions studied by Bally et al. 2015. Green pin is collection site of LAB accession.
Benthi — the workhorse of plant biology. Image art by Hsuan Pai. Adapted from Figure 1 of Derevnina et al. 2019.
Benthi is particularly appropriate in the post-genomics era, for example to perform high-throughput screens to connect plant pathogen effectors to biological activities. Adapted from Pais et al. 2013.
Co-expression of effectors from the plant pathogen Phytophthora infestans with the potato plant immune receptors Rpi-blb1 and Rpi-blb2 reveal the effectors that are detected by these immune receptors. Each effector is expressed in triplicate. Immune response is the dark hypersensitive cell death around the incoulation site. Source: Oh et al. 2009.
The potato immune receptor (aka R gene) Rpi-blb2 confers resistance to Phytophthora infestans (left leaf) when expressed in Nicotiana benthamiana. The leaf on the right is a control showing the infection lesions caused by the pathogen. Source: Kamoun Lab.
Fast-forward cell biology of the plant-pathogen interface. The image shows the pathogen hypha in red and its protruding haustoria that are invading the plant cell cavity and surrounded by the Remirin 1.3 plant protein. Adapted from Bozkurt et al. 2014. For more information, see Bozkurt and Kamoun 2020.
Nicotiana benthamiana (sensu lato) in its wild habitat in Western Australia. Photo credit: Maarten Christenhusz. Source: Royal Botanical Gardens Kew.

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